abstract：:Marsupial mammals show extraordinary karyotype stability, with 2n = 14 considered ancestral. However, macropodid marsupials (kangaroos and wallabies) exhibit a considerable variety of karyotypes, with a hypothesised ancestral karyotype of 2n = 22. Speciation and karyotypic diversity in rock wallabies (Petrogale) is ex...

journal_title：Genome

authors： O'Neill RJ,Eldridge MD,Toder R,Ferguson-Smith MA,O'Brien PC,Graves JA

更新日期：1999-06-01 00:00:00

abstract：:Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), w...

journal_title：Genome

authors： Sun GL,Díaz O,Salomon B,von Bothmer R

更新日期：1999-06-01 00:00:00

abstract：:Pigeon spermatocytes were processed with a drying-down technique and their synaptonemal complex (SC) complements were analyzed by electron microscopy. The synaptonemal complex karyotype of the macrobivalents shows an excellent correspondence with the mitotic karyotype. The number and distribution of recombination nodu...

journal_title：Genome

authors： Pigozzi MI,Solari AJ

更新日期：1999-04-01 00:00:00

abstract：:A novel tandemly repeated DNA structure of Toxoplasma gondii that meets the requirements assigned for satellital DNA was characterized. A DNA fragment of 1002 bp contains two different elements of repetitive DNA families named ABGTg7 and ABGTg8.2. Both repeats are members of a more complex tandem structure where ABGTg...

journal_title：Genome

authors： Matrajt M,Angel SO,Pszenny V,Guarnera E,Roos DS,Garberi JC

更新日期：1999-04-01 00:00:00

abstract：:Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequenc...

journal_title：Genome

authors： Rivkin MI,Vallejos CE,McClean PE

更新日期：1999-02-01 00:00:00

abstract：:We tested for complementation between two Mendelian mutations in mice, Far (first arch) and lgGa (lidgap-Gates). Each of these mutations gives greater than 70% risk of the birth defect, open eyelids, in homozygotes and gives little or no risk in heterozygotes. Far and lgGa are known to not be alleles; Far maps to Chr ...

journal_title：Genome

authors： Harris MJ,Juriloff DM

更新日期：1998-12-01 00:00:00

abstract：:Our goal was to purify and characterize the allelic variants H1b1 and H1b2 of histone H1.b, one of the seven subtypes of this linker histone extracted from Japanese quail erythrocyte nuclei. These variants are revealed phenotypically as band H1.3 or part of band H1.4 by polyacrylamide gel electrophoresis (PAGE) in sod...

journal_title：Genome

authors： Pałyga J,Neelin JM

更新日期：1998-10-01 00:00:00

abstract：:The abundance, genomic organization, species distribution, and structure of 33 distinct families of repetitive DNA in Phytophthora are described. The families were identified by screening a library of Phytophthora infestans DNA for repetitive sequences. These was subsequently characterized within 26 species distribute...

journal_title：Genome

authors： Judelson HS,Randall TA

更新日期：1998-08-01 00:00:00

abstract：:The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capit...

journal_title：Genome

authors： Zhao JT,Frommer M,Sved JA,Zacharopoulou A

更新日期：1998-08-01 00:00:00

abstract：:The genus Alstroemeria contains species with large genomes (2C = 36.5-78.9 pg (17,600-38,000 Mb) in those species with 2n = 2x = 16). We investigated the diversity and genomic and chromosomal organisation of Ty1-copia-like retrotransposons in four Alstroemeria species. Analysis of 33 PCR-amplified sequences correspond...

journal_title：Genome

authors： Kuipers AG,Heslop-Harrison JS,Jacobsen E

更新日期：1998-06-01 00:00:00

abstract：:Insulin-like growth factor 1 (IGF1) regulates growth and metabolic functions in vertebrates. A dinucleotide repeat sequence located at the promoter region of the IGF1 gene has been reported in several vertebrate species and may affect the control of the transcriptional activity of this gene. The genotypes of animals f...

journal_title：Genome

authors： Caetano AR,Bowling AT

更新日期：1998-02-01 00:00:00

abstract：:The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...

journal_title：Genome

authors： Arndt GM,Rank GH

更新日期：1997-12-01 00:00:00

abstract：:The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...

journal_title：Genome

authors： Sola L,Marzovillo M,Rossi AR,Gornung E,Bressanello S,Turner BJ

更新日期：1997-12-01 00:00:00

abstract：:Polycelis nigra is a free-living simultaneous hermaphroditic flatworm that has amphimictic and pseudogamous parthenogenetic biotypes. Sexual individuals are always diploid (2n = 16) and pseudogamous parthenogens are polyploid (usually triploid). Two types of supernumerary chromosomes are found in parthenogens, those r...

journal_title：Genome

authors： Sharbel TF,Beukeboom LW,Pijnacker LP

更新日期：1997-12-01 00:00:00

abstract：:Digestion of Rana graeca italica DNA with Asp 718I produces highly repetitive fragments of 281 and 385 bp that were cloned and sequenced. The shorter fragment corresponds to the unit repeat (RgiS1b) of a satellite DNA. The longer fragment was found to be part of a 494-bp repeat of another satellite DNA (RgiS1a) that w...

journal_title：Genome

authors： Cardone DE,Feliciello I,Marotta M,Rosati C,Chinali G

更新日期：1997-10-01 00:00:00

abstract：:Barley yellow dwarf virus (BYDV) resistance in soft red winter wheat (SRWW) cultivars has been achieved by substituting a group 7 chromosome from Thinopyrum intermedium for chromosome 7D. To localize BYDV resistance, a detailed molecular genetic analysis was done on the alien group 7 Th. intermedium chromosome to dete...

journal_title：Genome

authors： Francki MG,Crasta OR,Sharma HC,Ohm HW,Anderson JM

更新日期：1997-10-01 00:00:00

abstract：:Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...

journal_title：Genome

authors： Pedersen C,Langridge P

更新日期：1997-10-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads...

journal_title：Genome

authors： Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RA

更新日期：1997-08-01 00:00:00

abstract：:Doubled haploid (DH) lines together with a cotyledon bioassay were employed for the molecular analysis of resistance to the blackleg fungus Leptosphaeria maculans in the Australian Brassica napus cultivars Shiralee and Maluka. We used bulked segregant analysis to identify 13 RAPD and two RFLP markers linked to the res...

journal_title：Genome

authors： Mayerhofer R,Good AG,Bansal VK,Thiagarajah MR,Stringam GR

更新日期：1997-06-01 00:00:00

abstract：:Alien cytoplasms cause a wide range of phenotypic alterations in the nucleus-cytoplasm (NC) hybrids in the Triticeae. Nuclear genomes of timopheevii wheat (Triticum timopheevii and Triticum araraticum) are fully compatible with the cytoplasm of Aegilops squarrosa, while those of a majority of emmer or durum wheat cult...

journal_title：Genome

authors： Asakura N,Nakamura C,Ohtsuka I

更新日期：1997-04-01 00:00:00

abstract：:After temperature elevation, the newly synthesized polypeptides from several Drosophila montium subgroup species, of the melanogaster species group, were analyzed in denaturing acrylamide gels. The pattern obtained is characteristic of the heat shock response already documented for many other Drosophila species, altho...

journal_title：Genome

authors： Konstantopoulou I,Drosopoulou E,Scouras ZG

更新日期：1997-02-01 00:00:00

abstract：:A high-resolution G-banding technique was used to identify five metacentrics that characterize Suncus murinus from Sri Lanka. These metacentrics were shown to be the product of Robertsonian fusion of acrocentric chromosomes identical to those in the standard karyotype defined by M.B. Rogatcheva et al. Two of the metac...

journal_title：Genome

authors： Rogatcheva MB,Borodin PM,Oda SI,Searle JB

更新日期：1997-02-01 00:00:00

abstract：:Using genomic in situ hybridization to chromosomes, we identified introgressed segments in a tetrageneric hybrid of Triticum, Avena, Thinopyrum, and Secale, which conferred high resistance to leaf rust, stem rust, stripe rust, powdery mildew, and root rot to wheat. The disease-resistance traits of the hybrid originate...

journal_title：Genome

authors： Tang S,Zhuang J,Wen Y,Ai SA,Li H,Xu J

更新日期：1997-02-01 00:00:00

abstract：:An F2 progeny of maritime pine (Pinus pinaster Aïton) was used to investigate the mode of inheritance of δ3-carene using a quantitative and a qualitative approach. A previously reported genetic map constructed with random amplified polymorphic DNA (RAPD) markers made it possible to locate one major quantitative ...

journal_title：Genome

authors： Plomion C,Yani A,Marpeau A

更新日期：1996-12-01 00:00:00

abstract：:Three wheat sequences, shown to be homologous to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes were localized on chromosomes using nullisomic-tetrasomic wheat ('Chinese Spring') and wheat-rye ('Chinese Spring'/'Imperial') addition lines. Whereas the loci for the pol...

journal_title：Genome

authors： Korzun V,Balzer HJ,Balzer A,Bäumlein H,Börner A

更新日期：1996-12-01 00:00:00

abstract：:Distribution, organization, and molecular analysis of four unrelated satellite DNA components in Aveneae species are described. Highly repeated DNA elements were cloned from Helictotrichon convolutum (CON1 and CON2) and Helictotrichon compressum (COM1 and COM2). The lengths of the repeat monomers are 365 bp (CON1), 56...

journal_title：Genome

authors： Grebenstein B,Grebenstein O,Sauer W,Hemleben V

更新日期：1996-12-01 00:00:00

abstract：:This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...

journal_title：Genome

authors： Yowe DL,Epping RJ

更新日期：1996-10-01 00:00:00

abstract：:Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...

journal_title：Genome

authors： Prins R,Marais GF,Marais AS,Janse BJ,Pretorius ZA

更新日期：1996-10-01 00:00:00

abstract：:An analysis of the effect of changing physiological conditions on genome evolution in tomato cell populations has been carried out on long-term in vitro cultured clones grown on different auxin-cytokinin equilibria or selected for low-high competence for active defense against Fusarium oxysporum f.sp. lycopersici. RAP...

journal_title：Genome

authors： Bogani P,Simoni A,Lio' P,Scialpi A,Buiatti M

更新日期：1996-10-01 00:00:00

abstract：:The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp ...

journal_title：Genome

authors： Williams KJ,Fisher JM,Langridge P

更新日期：1996-08-01 00:00:00

abstract：:Molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. BN28 is a low temperature induced gene in Brassicaceae species. PCR and Southern blot analysis indicate that BN28 is polymorphic in the three diploid genomes: Brassica rapa (AA), Brassica nigra (BB), and B...

journal_title：Genome

authors： Hawkins GP,Nykiforuk CL,Johnson-Flanagan AM,Boothe JG

更新日期：1996-08-01 00:00:00

abstract：:Comparative RFLP linkage maps were constructed using five segregating populations derived from two self-incompatible lines (termed PI 230582 and PI 458314) of diploid tuber-bearing Solanum chacoense Bitt. The analysis was based on 84 RFLP loci identified by 73 different cDNA clones. Distortion of expected Mendelian se...

journal_title：Genome

authors： Rivard SR,Cappadocia M,Landry BS

更新日期：1996-08-01 00:00:00

abstract：:Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, y...

journal_title：Genome

authors： Liharska T,Wordragen M,Kammen A,Zabel P,Koornneef M

更新日期：1996-06-01 00:00:00

abstract：:The POSCH-2 Y chromosome from the poschiavinus variety of Mus musculus domesticus causes incomplete testis development in the recessive autosomal background of the C57BL/6J laboratory mouse strain. Testis development is normal with the POSCH-2 Y in its native strain background as well as in some strains of the laborat...

journal_title：Genome

authors： Eales BA,Nahas M,Biddle FG

更新日期：1996-06-01 00:00:00

abstract：:Canada wild rye (CWR, Elymus canadensis L., 2n = 4x = 28) is a potential source of genes for disease resistance and environmental tolerance in barley (Hordeum vulgare L., 2n = 2x = 14). Tissue cultures were initiated from immature inflorescences of CWR x 'Betzes' barley hybrids to promote CWR introgression into barley...

journal_title：Genome

authors： Dahleen LS

更新日期：1996-04-01 00:00:00

abstract：:The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reac...

journal_title：Genome

authors： El-Kharbotly A,Jacobs JM,Hekkert BT,Stiekema WJ,Pereira A,Jacobsen E,Ramanna MS

更新日期：1996-04-01 00:00:00

abstract：:A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification th...

journal_title：Genome

authors： Cai Q,Guy CL,Moore GA

更新日期：1996-04-01 00:00:00

abstract：:Lycopersicon esculentum (tomato) has a small genome (2C = 1.90 pg of DNA) packaged in 2n = 2x = 24 small acrocentric to metacentric chromosomes. Like the chromosomes of other members of the family Solanaceae, tomato chromosomes have pericentromeric heterochromatin. To determine the fraction of the tomato genome found ...

journal_title：Genome

authors： Peterson DG,Stack SM,Price HJ,Johnston JS

更新日期：1996-02-01 00:00:00

abstract：:In combination with oligonucleotides of arbitrary sequence, 5' anchored oligonucleotides based on simple sequence repeats were used in polymerase chain reaction amplifications to produce barley DNA fingerprints. The aim of this work was (i) to develop a simple nonradioactive experimental procedure to reveal polymorphi...

journal_title：Genome

authors： Sánchez de la Hoz MP,Dávila JA,Loarce Y,Ferrer E

更新日期：1996-02-01 00:00:00

abstract：:DNA repeats, revealed initially by digestion of horse DNA with restriction enzymes, were cloned and characterized by cross-hybridization studies and nucleotide sequencing. The Sau-like family of tandem repeats contained two classes of repetitive elements with unit repeats of about 80 bp that shared no sequence similar...

journal_title：Genome

authors： Broad TE,Ede AJ,Forrest JW,Lewis PE,Phua SH,Pugh PA

更新日期：1995-12-01 00:00:00